Device

Part:BBa_K176067:Design

Designed by: Zongxiao He, Hao Jiang   Group: iGEM09_USTC   (2009-07-30)


aTc&AHL->GFP: pCon 0.70->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 898
    Illegal NheI site found at 921
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1876
    Illegal BsaI.rc site found at 2620


Design Notes

Input1: aTc

Input2: AHL

Output: GFP

Design:

pCon 0.70(J23101) generates TetR-LVA

pCon 0.70(J23101) generates LuxR

pLux/Tet(K176000) generates GFP, activated by LuxR and Input2 AHL, repressed by TetR-LVA and no Input1 aTc


Source

J23101 K176030

References